Virus:Article Title: Multifunctional carboxymethyl cellulose -based hydrogels with zwitterionic and silver nanowire components for wound management
Article Snippet: 794 795 796 STAR★METHODS 797 KEY RESOURCES TABLE 798 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 antibody Servicebio Cat.No. .. : GB15063-100 Anti-Ki67 primary antibody Servicebio Cat.No.:GB1211 41-100 Anti-CD68 antibody Servicebio Cat.No.: GB153150-100 Anti-CD163 antibody Servicebio Cat.No.: GB15340-100 Secondary antibodies conjugated with Alexa Fluor 488 Jackson ImmunoResearch Cat# 715-545-150 Jo urn al Pr e-p roo f 31 Secondary antibodies conjugated with Alexa Fluor 594 Jackson ImmunoResearch Cat# 715-585-150 Bacterial and virus strains Escherichia coli American Type Culture Collection ATCC 25922 Staphylococcus aureus American Type Culture Collection ATCC 25923 Chemicals, peptides, and recombinant proteins 4-vinylpyridine Merck Cat# V3204 β-butyrolactone Merck Cat# 219126 1,3-propane sultone Merck Cat# P50706 N-[3-(dimethylamino)propyl]methacrylamide Merck Cat# 409472 Acrylic acid Merck Cat# 147230 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) Merck Cat# E6383 J urn al Pr e-p roo f 32 4-dimethylaminopyridine (DMAP) Merck Cat# 107700 Silver nanowires Merck Cat# 778095 Ammonium persulfate (APS) Merck Cat# A3678 Tetramethylethylenediamine(TMEDA) Merck Cat# 411019 Dulbecco’s modified eagle medium(DMEM) Gibco Cat# 11965092 Reagent [3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulphophenyl)-2Htetrazolium] (MTS) Promega Cat# G3580 2,2-diphenyl-1-picrylhydrazyl (DPPH) Sigma-Aldrich Cat# 300267 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) Sigma-Aldrich Cat# 194430 Penicillin-streptomycin Gibco 15140122 Trypsin-EDTA Gibco 25200072 Fetal bovine serum (FBS) Gibco A5256501 Experimental models: Cell lines Jo urn al Pr e-p roo f 33 Mouse fibroblast cell line (L929) Shanghai Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Male KM mice, 6–8 weeks old Zhuhai BesTest Bio- Tech Co., Ltd. N/A Software and algorithms Grapad Prism 9.3 GraphPad Software https://www.graph pad-prism.cn/ Image J National Institutes of Health https://imagej.nih.g ov/ij/ Image-Pro Plus 7.1 Media Cybernetics https://my.mediacy .com/support/updat es Figdraw Home for Researchers https://www.figdra w.com 799 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 800 In this experiment, L929 cell line, E. Coli and S. aureus were used for the cytocompatibility 801 experiment and antibacterial performance of hydrogels, respectively. ..
Recombinant:Article Title: Multifunctional carboxymethyl cellulose -based hydrogels with zwitterionic and silver nanowire components for wound management
Article Snippet: 794 795 796 STAR★METHODS 797 KEY RESOURCES TABLE 798 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 antibody Servicebio Cat.No. .. : GB15063-100 Anti-Ki67 primary antibody Servicebio Cat.No.:GB1211 41-100 Anti-CD68 antibody Servicebio Cat.No.: GB153150-100 Anti-CD163 antibody Servicebio Cat.No.: GB15340-100 Secondary antibodies conjugated with Alexa Fluor 488 Jackson ImmunoResearch Cat# 715-545-150 Jo urn al Pr e-p roo f 31 Secondary antibodies conjugated with Alexa Fluor 594 Jackson ImmunoResearch Cat# 715-585-150 Bacterial and virus strains Escherichia coli American Type Culture Collection ATCC 25922 Staphylococcus aureus American Type Culture Collection ATCC 25923 Chemicals, peptides, and recombinant proteins 4-vinylpyridine Merck Cat# V3204 β-butyrolactone Merck Cat# 219126 1,3-propane sultone Merck Cat# P50706 N-[3-(dimethylamino)propyl]methacrylamide Merck Cat# 409472 Acrylic acid Merck Cat# 147230 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) Merck Cat# E6383 J urn al Pr e-p roo f 32 4-dimethylaminopyridine (DMAP) Merck Cat# 107700 Silver nanowires Merck Cat# 778095 Ammonium persulfate (APS) Merck Cat# A3678 Tetramethylethylenediamine(TMEDA) Merck Cat# 411019 Dulbecco’s modified eagle medium(DMEM) Gibco Cat# 11965092 Reagent [3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulphophenyl)-2Htetrazolium] (MTS) Promega Cat# G3580 2,2-diphenyl-1-picrylhydrazyl (DPPH) Sigma-Aldrich Cat# 300267 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) Sigma-Aldrich Cat# 194430 Penicillin-streptomycin Gibco 15140122 Trypsin-EDTA Gibco 25200072 Fetal bovine serum (FBS) Gibco A5256501 Experimental models: Cell lines Jo urn al Pr e-p roo f 33 Mouse fibroblast cell line (L929) Shanghai Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Male KM mice, 6–8 weeks old Zhuhai BesTest Bio- Tech Co., Ltd. N/A Software and algorithms Grapad Prism 9.3 GraphPad Software https://www.graph pad-prism.cn/ Image J National Institutes of Health https://imagej.nih.g ov/ij/ Image-Pro Plus 7.1 Media Cybernetics https://my.mediacy .com/support/updat es Figdraw Home for Researchers https://www.figdra w.com 799 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 800 In this experiment, L929 cell line, E. Coli and S. aureus were used for the cytocompatibility 801 experiment and antibacterial performance of hydrogels, respectively. ..
Modification:Article Title: Multifunctional carboxymethyl cellulose -based hydrogels with zwitterionic and silver nanowire components for wound management
Article Snippet: 794 795 796 STAR★METHODS 797 KEY RESOURCES TABLE 798 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 antibody Servicebio Cat.No. .. : GB15063-100 Anti-Ki67 primary antibody Servicebio Cat.No.:GB1211 41-100 Anti-CD68 antibody Servicebio Cat.No.: GB153150-100 Anti-CD163 antibody Servicebio Cat.No.: GB15340-100 Secondary antibodies conjugated with Alexa Fluor 488 Jackson ImmunoResearch Cat# 715-545-150 Jo urn al Pr e-p roo f 31 Secondary antibodies conjugated with Alexa Fluor 594 Jackson ImmunoResearch Cat# 715-585-150 Bacterial and virus strains Escherichia coli American Type Culture Collection ATCC 25922 Staphylococcus aureus American Type Culture Collection ATCC 25923 Chemicals, peptides, and recombinant proteins 4-vinylpyridine Merck Cat# V3204 β-butyrolactone Merck Cat# 219126 1,3-propane sultone Merck Cat# P50706 N-[3-(dimethylamino)propyl]methacrylamide Merck Cat# 409472 Acrylic acid Merck Cat# 147230 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) Merck Cat# E6383 J urn al Pr e-p roo f 32 4-dimethylaminopyridine (DMAP) Merck Cat# 107700 Silver nanowires Merck Cat# 778095 Ammonium persulfate (APS) Merck Cat# A3678 Tetramethylethylenediamine(TMEDA) Merck Cat# 411019 Dulbecco’s modified eagle medium(DMEM) Gibco Cat# 11965092 Reagent [3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulphophenyl)-2Htetrazolium] (MTS) Promega Cat# G3580 2,2-diphenyl-1-picrylhydrazyl (DPPH) Sigma-Aldrich Cat# 300267 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) Sigma-Aldrich Cat# 194430 Penicillin-streptomycin Gibco 15140122 Trypsin-EDTA Gibco 25200072 Fetal bovine serum (FBS) Gibco A5256501 Experimental models: Cell lines Jo urn al Pr e-p roo f 33 Mouse fibroblast cell line (L929) Shanghai Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Male KM mice, 6–8 weeks old Zhuhai BesTest Bio- Tech Co., Ltd. N/A Software and algorithms Grapad Prism 9.3 GraphPad Software https://www.graph pad-prism.cn/ Image J National Institutes of Health https://imagej.nih.g ov/ij/ Image-Pro Plus 7.1 Media Cybernetics https://my.mediacy .com/support/updat es Figdraw Home for Researchers https://www.figdra w.com 799 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 800 In this experiment, L929 cell line, E. Coli and S. aureus were used for the cytocompatibility 801 experiment and antibacterial performance of hydrogels, respectively. ..
Software:Article Title: Multifunctional carboxymethyl cellulose -based hydrogels with zwitterionic and silver nanowire components for wound management
Article Snippet: 794 795 796 STAR★METHODS 797 KEY RESOURCES TABLE 798 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD31 antibody Servicebio Cat.No. .. : GB15063-100 Anti-Ki67 primary antibody Servicebio Cat.No.:GB1211 41-100 Anti-CD68 antibody Servicebio Cat.No.: GB153150-100 Anti-CD163 antibody Servicebio Cat.No.: GB15340-100 Secondary antibodies conjugated with Alexa Fluor 488 Jackson ImmunoResearch Cat# 715-545-150 Jo urn al Pr e-p roo f 31 Secondary antibodies conjugated with Alexa Fluor 594 Jackson ImmunoResearch Cat# 715-585-150 Bacterial and virus strains Escherichia coli American Type Culture Collection ATCC 25922 Staphylococcus aureus American Type Culture Collection ATCC 25923 Chemicals, peptides, and recombinant proteins 4-vinylpyridine Merck Cat# V3204 β-butyrolactone Merck Cat# 219126 1,3-propane sultone Merck Cat# P50706 N-[3-(dimethylamino)propyl]methacrylamide Merck Cat# 409472 Acrylic acid Merck Cat# 147230 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) Merck Cat# E6383 J urn al Pr e-p roo f 32 4-dimethylaminopyridine (DMAP) Merck Cat# 107700 Silver nanowires Merck Cat# 778095 Ammonium persulfate (APS) Merck Cat# A3678 Tetramethylethylenediamine(TMEDA) Merck Cat# 411019 Dulbecco’s modified eagle medium(DMEM) Gibco Cat# 11965092 Reagent [3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulphophenyl)-2Htetrazolium] (MTS) Promega Cat# G3580 2,2-diphenyl-1-picrylhydrazyl (DPPH) Sigma-Aldrich Cat# 300267 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) Sigma-Aldrich Cat# 194430 Penicillin-streptomycin Gibco 15140122 Trypsin-EDTA Gibco 25200072 Fetal bovine serum (FBS) Gibco A5256501 Experimental models: Cell lines Jo urn al Pr e-p roo f 33 Mouse fibroblast cell line (L929) Shanghai Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Male KM mice, 6–8 weeks old Zhuhai BesTest Bio- Tech Co., Ltd. N/A Software and algorithms Grapad Prism 9.3 GraphPad Software https://www.graph pad-prism.cn/ Image J National Institutes of Health https://imagej.nih.g ov/ij/ Image-Pro Plus 7.1 Media Cybernetics https://my.mediacy .com/support/updat es Figdraw Home for Researchers https://www.figdra w.com 799 EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 800 In this experiment, L929 cell line, E. Coli and S. aureus were used for the cytocompatibility 801 experiment and antibacterial performance of hydrogels, respectively. ..
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